■ 基本信息
啟動子: | T7 |
復制子: | pBR322 |
質粒分類: | 大腸桿菌載體；克隆質粒 |
質粒大小: | 2464bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
5'測序引物: | Sp6:ATTTAGGTGACACTATAGAA |
3'測序引物: | T7:TAATACGACTCACTATAGGG |
備注: | 豐富的酶切位點 |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 基因模板 |
片段類型: | cDNA
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
The pSP73 Vector offers a wide range of restriction sites, providing greater versatility in cloning and transcription of RNA in vitro. The pSP73 Vector contains the SP6 and T7 RNA polymerase promoters and a unique multiple cloning region, which includes restriction sites for BglII, EcoRV, ClaI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII, PvuII and XhoI. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning region.
質粒只保證關鍵序列正確，不保證表達效果。
■ 質粒圖譜
■ 質粒序列
質粒序列可參考（具體以測序為準）下載：
ZK123pSP73體外轉錄質粒.txt
